Interaction Details


Details of experiments between P61981 and O14639
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 350-359
Site: 14-3-3_pSer355
Modification: (SEP)355
Sequence: RTSSESIYSR
Construct: RTSSE(pSer)IYSR (crude synthetic peptide)
Average holdup BI: 0.09
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 134
P value: 1.19
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 350-359
Site: 14-3-3_pSer355
Modification: (SEP)355
Sequence: RTSSESIYSR
Construct: RTSSE(pSer)IYSR (crude synthetic peptide)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 129
P value: 1.1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 350-359
Site: 14-3-3_pSer355
Modification: (SEP)355
Sequence: RTSSESIYSR
Construct: RTSSE(pSer)IYSR (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 115
P value: 0.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 353-362
Site: 14-3-3_pSer358
Modification: (SEP)358
Sequence: SESIYSRPGS
Construct: SESIY(pSer)RPGS (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 134
P value: 0.08
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 353-362
Site: 14-3-3_pSer358
Modification: (SEP)358
Sequence: SESIYSRPGS
Construct: SESIY(pSer)RPGS (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 129
P value: 0.12
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 353-362
Site: 14-3-3_pSer358
Modification: (SEP)358
Sequence: SESIYSRPGS
Construct: SESIY(pSer)RPGS (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 115
P value: 0.26
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 358-367
Site: 14-3-3_pSer363
Modification: (SEP)363
Sequence: SRPGSSIPGS
Construct: SRPGS(pSer)IPGS (crude synthetic peptide)
Average holdup BI: 0.1
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 134
P value: 2.49
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 358-367
Site: 14-3-3_pSer363
Modification: (SEP)363
Sequence: SRPGSSIPGS
Construct: SRPGS(pSer)IPGS (crude synthetic peptide)
Average holdup BI: 0.11
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 129
P value: 3.63
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 358-367
Site: 14-3-3_pSer363
Modification: (SEP)363
Sequence: SRPGSSIPGS
Construct: SRPGS(pSer)IPGS (crude synthetic peptide)
Average holdup BI: 0.33
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 115
P value: 6.75
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.65
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 34-43
Site: 14-3-3_pSer39
Modification: (SEP)39
Sequence: NIRRSSSDFF
Construct: NIRRS(pSer)SDFF (crude synthetic peptide)
Average holdup BI: 0.24
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 134
P value: 3.42
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 11

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 34-43
Site: 14-3-3_pSer39
Modification: (SEP)39
Sequence: NIRRSSSDFF
Construct: NIRRS(pSer)SDFF (crude synthetic peptide)
Average holdup BI: 0.28
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 129
P value: 4.17
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 12

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 34-43
Site: 14-3-3_pSer39
Modification: (SEP)39
Sequence: NIRRSSSDFF
Construct: NIRRS(pSer)SDFF (crude synthetic peptide)
Average holdup BI: 0.21
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 115
P value: 4.46
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 13

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 417-426
Site: 14-3-3_pSer422
Modification: (SEP)422
Sequence: KQERQSLGES
Construct: KQERQ(pSer)LGES (crude synthetic peptide)
Average holdup BI: 0.2
Holdup BI standard deviation: 0.73
Immobilized partner concentration (10-6M): 134
P value: 0.15
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 14

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 430-439
Site: 14-3-3_pSer435
Modification: (SEP)435
Sequence: LSPTPSAEGY
Construct: LSPTP(pSer)AEGY (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 134
P value: 0.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 15

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 430-439
Site: 14-3-3_pSer435
Modification: (SEP)435
Sequence: LSPTPSAEGY
Construct: LSPTP(pSer)AEGY (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 129
P value: 0.26
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 16

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 430-439
Site: 14-3-3_pSer435
Modification: (SEP)435
Sequence: LSPTPSAEGY
Construct: LSPTP(pSer)AEGY (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.14
Immobilized partner concentration (10-6M): 115
P value: 0.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 17

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 447-456
Site: 14-3-3_pSer452
Modification: (SEP)452
Sequence: IHRSTSQGSI
Construct: IHRST(pSer)QGSI (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 134
P value: 0.06
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 18

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 447-456
Site: 14-3-3_pSer452
Modification: (SEP)452
Sequence: IHRSTSQGSI
Construct: IHRST(pSer)QGSI (crude synthetic peptide)
Average holdup BI: -0.35
Holdup BI standard deviation: 0.67
Immobilized partner concentration (10-6M): 129
P value: 0.69
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 19

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 447-456
Site: 14-3-3_pSer452
Modification: (SEP)452
Sequence: IHRSTSQGSI
Construct: IHRST(pSer)QGSI (crude synthetic peptide)
Average holdup BI: -0.19
Holdup BI standard deviation: 0.27
Immobilized partner concentration (10-6M): 115
P value: 0.72
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 20

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 457-466
Site: 14-3-3_pSer462
Modification: (SEP)462
Sequence: NSPVYSRHSY
Construct: NSPVY(pSer)RHSY (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 134
P value: 0.6
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 21

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 457-466
Site: 14-3-3_pSer462
Modification: (SEP)462
Sequence: NSPVYSRHSY
Construct: NSPVY(pSer)RHSY (crude synthetic peptide)
Average holdup BI: -0.08
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 129
P value: 0.71
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 22

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 457-466
Site: 14-3-3_pSer462
Modification: (SEP)462
Sequence: NSPVYSRHSY
Construct: NSPVY(pSer)RHSY (crude synthetic peptide)
Average holdup BI: 0.05
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 115
P value: 0.38
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 23

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 581-590
Site: 14-3-3_pSer586
Modification: (SEP)586
Sequence: DMKRRSSGRE
Construct: DMKRR(pSer)SGRE (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.28
Immobilized partner concentration (10-6M): 115
P value: 0.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 24

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 650-659
Site: 14-3-3_pSer655
Modification: (SEP)655
Sequence: SSKTASLPGY
Construct: SSKTA(pSer)LPGY (crude synthetic peptide)
Average holdup BI: 0.87
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 129
P value: 9.99
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.73
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 25

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 650-659
Site: 14-3-3_pSer655
Modification: (SEP)655
Sequence: SSKTASLPGY
Construct: SSKTA(pSer)LPGY (crude synthetic peptide)
Average holdup BI: 0.85
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 134
P value: 9.44
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.63
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 26

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 650-659
Site: 14-3-3_pSer655
Modification: (SEP)655
Sequence: SSKTASLPGY
Construct: SSKTA(pSer)LPGY (crude synthetic peptide)
Average holdup BI: 0.82
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 115
P value: 7.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.6
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 27

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 664-673
Site: 14-3-3_pSer669
Modification: (SEP)669
Sequence: LHRPVSTDFA
Construct: LHRPV(pSer)TDFA (crude synthetic peptide)
Average holdup BI: 0.11
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 134
P value: 1.59
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 28

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 664-673
Site: 14-3-3_pSer669
Modification: (SEP)669
Sequence: LHRPVSTDFA
Construct: LHRPV(pSer)TDFA (crude synthetic peptide)
Average holdup BI: 0.08
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 129
P value: 1.48
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 29

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 664-673
Site: 14-3-3_pSer669
Modification: (SEP)669
Sequence: LHRPVSTDFA
Construct: LHRPV(pSer)TDFA (crude synthetic peptide)
Average holdup BI: 0.14
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 115
P value: 2.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 30

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 701-710
Site: 14-3-3_pSer706
Modification: (SEP)706
Sequence: MDRGVSMPNM
Construct: MDRGV(pSer)MPNM (crude synthetic peptide)
Average holdup BI: 0.96
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 129
P value: 7.17
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.25
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 31

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 701-710
Site: 14-3-3_pSer706
Modification: (SEP)706
Sequence: MDRGVSMPNM
Construct: MDRGV(pSer)MPNM (crude synthetic peptide)
Average holdup BI: 0.95
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 134
P value: 2.82
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 5.15
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 32

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 701-710
Site: 14-3-3_pSer706
Modification: (SEP)706
Sequence: MDRGVSMPNM
Construct: MDRGV(pSer)MPNM (crude synthetic peptide)
Average holdup BI: 0.88
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 115
P value: 2.75
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.81
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 33

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 366-375
Site: 14-3-3_pThr371
Modification: (TPO)371
Sequence: GSPGHTIYAK
Construct: GSPGH(pThr)IYAK (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 134
P value: 0.63
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 34

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 366-375
Site: 14-3-3_pThr371
Modification: (TPO)371
Sequence: GSPGHTIYAK
Construct: GSPGH(pThr)IYAK (crude synthetic peptide)
Average holdup BI: -0.03
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 129
P value: 0.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 35

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 366-375
Site: 14-3-3_pThr371
Modification: (TPO)371
Sequence: GSPGHTIYAK
Construct: GSPGH(pThr)IYAK (crude synthetic peptide)
Average holdup BI: -0.06
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 115
P value: 0.32
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 36

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 424-433
Site: 14-3-3_pThr429
Modification: (TPO)429
Sequence: GESPRTLSPT
Construct: GESPR(pThr)LSPT (crude synthetic peptide)
Average holdup BI: 0.17
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 134
P value: 4.37
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 37

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 424-433
Site: 14-3-3_pThr429
Modification: (TPO)429
Sequence: GESPRTLSPT
Construct: GESPR(pThr)LSPT (crude synthetic peptide)
Average holdup BI: 0.17
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 129
P value: 5.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 38

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: ABLIM1
Fragment: 424-433
Site: 14-3-3_pThr429
Modification: (TPO)429
Sequence: GESPRTLSPT
Construct: GESPR(pThr)LSPT (crude synthetic peptide)
Average holdup BI: 0.13
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 115
P value: 3.35
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))