Interaction Details


Details of experiments between P61981 and Q6UUV9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CRTC1
Fragment: 146-155
Site: 14-3-3_pSer151
Modification: (SEP)151
Sequence: WRRTNSDSAL
Construct: WRRTN(pSer)DSAL (crude synthetic peptide)
Average holdup BI: 0.89
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 134
P value: 8.83
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.8
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CRTC1
Fragment: 146-155
Site: 14-3-3_pSer151
Modification: (SEP)151
Sequence: WRRTNSDSAL
Construct: WRRTN(pSer)DSAL (crude synthetic peptide)
Average holdup BI: 0.88
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 129
P value: 10.22
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.76
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CRTC1
Fragment: 146-155
Site: 14-3-3_pSer151
Modification: (SEP)151
Sequence: WRRTNSDSAL
Construct: WRRTN(pSer)DSAL (crude synthetic peptide)
Average holdup BI: 0.93
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 115
P value: 7.1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 5.1
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CRTC1
Fragment: 183-192
Site: 14-3-3_pSer188
Modification: (SEP)188
Sequence: SFSSGSQDVH
Construct: SFSSG(pSer)QDVH (crude synthetic peptide)
Average holdup BI: -0.09
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 134
P value: 1.59
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CRTC1
Fragment: 183-192
Site: 14-3-3_pSer188
Modification: (SEP)188
Sequence: SFSSGSQDVH
Construct: SFSSG(pSer)QDVH (crude synthetic peptide)
Average holdup BI: -0.07
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 129
P value: 1.6
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CRTC1
Fragment: 183-192
Site: 14-3-3_pSer188
Modification: (SEP)188
Sequence: SFSSGSQDVH
Construct: SFSSG(pSer)QDVH (crude synthetic peptide)
Average holdup BI: -0.07
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 115
P value: 2.25
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))