Interaction Details


Details of experiments between P61981 and Q99759
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 142-151
Site: 14-3-3_pSer147
Modification: (SEP)147
Sequence: IKASQSAGDI
Construct: IKASQ(pSer)AGDI (crude synthetic peptide)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 134
P value: 0.35
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 142-151
Site: 14-3-3_pSer147
Modification: (SEP)147
Sequence: IKASQSAGDI
Construct: IKASQ(pSer)AGDI (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 129
P value: 0.35
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 142-151
Site: 14-3-3_pSer147
Modification: (SEP)147
Sequence: IKASQSAGDI
Construct: IKASQ(pSer)AGDI (crude synthetic peptide)
Average holdup BI: 0.01
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 115
P value: 0.12
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 161-170
Site: 14-3-3_pSer166
Modification: (SEP)166
Sequence: RSRHLSVSSQ
Construct: RSRHL(pSer)VSSQ (crude synthetic peptide)
Average holdup BI: 0.96
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 134
P value: 3.8
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 5.27
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 245-254
Site: 14-3-3_pSer250
Modification: (SEP)250
Sequence: MSRAQSFPDN
Construct: MSRAQ(pSer)FPDN (crude synthetic peptide)
Average holdup BI: 0.81
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 129
P value: 13.69
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.51
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 245-254
Site: 14-3-3_pSer250
Modification: (SEP)250
Sequence: MSRAQSFPDN
Construct: MSRAQ(pSer)FPDN (crude synthetic peptide)
Average holdup BI: 0.78
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 134
P value: 5.88
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.41
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 245-254
Site: 14-3-3_pSer250
Modification: (SEP)250
Sequence: MSRAQSFPDN
Construct: MSRAQ(pSer)FPDN (crude synthetic peptide)
Average holdup BI: 0.66
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 115
P value: 4.43
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.25
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 332-341
Site: 14-3-3_pSer337
Modification: (SEP)337
Sequence: RGRLRSADSE
Construct: RGRLR(pSer)ADSE (crude synthetic peptide)
Average holdup BI: 0.47
Holdup BI standard deviation: 0.2
Immobilized partner concentration (10-6M): 129
P value: 1.67
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.84
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 335-344
Site: 14-3-3_pSer340
Modification: (SEP)340
Sequence: LRSADSENAL
Construct: LRSAD(pSer)ENAL (crude synthetic peptide)
Average holdup BI: 0.07
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 134
P value: 3.31
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 335-344
Site: 14-3-3_pSer340
Modification: (SEP)340
Sequence: LRSADSENAL
Construct: LRSAD(pSer)ENAL (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 129
P value: 0.29
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 11

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 335-344
Site: 14-3-3_pSer340
Modification: (SEP)340
Sequence: LRSADSENAL
Construct: LRSAD(pSer)ENAL (crude synthetic peptide)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 115
P value: 0.87
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 12

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 352-361
Site: 14-3-3_pSer357
Modification: (SEP)357
Sequence: PTKSPSAPIN
Construct: PTKSP(pSer)APIN (crude synthetic peptide)
Average holdup BI: 0.85
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 129
P value: 8.57
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.64
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 13

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 352-361
Site: 14-3-3_pSer357
Modification: (SEP)357
Sequence: PTKSPSAPIN
Construct: PTKSP(pSer)APIN (crude synthetic peptide)
Average holdup BI: 0.84
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 134
P value: 8.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.58
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 14

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 352-361
Site: 14-3-3_pSer357
Modification: (SEP)357
Sequence: PTKSPSAPIN
Construct: PTKSP(pSer)APIN (crude synthetic peptide)
Average holdup BI: 0.78
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 115
P value: 7.05
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.51
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 15

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 289-298
Site: 14-3-3_pThr294
Modification: (TPO)294
Sequence: SDGRRTFPRI
Construct: SDGRR(pThr)FPRI (crude synthetic peptide)
Average holdup BI: 0.85
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 129
P value: 7.02
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.63
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 16

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 289-298
Site: 14-3-3_pThr294
Modification: (TPO)294
Sequence: SDGRRTFPRI
Construct: SDGRR(pThr)FPRI (crude synthetic peptide)
Average holdup BI: 0.78
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 134
P value: 6.06
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.42
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 17

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 289-298
Site: 14-3-3_pThr294
Modification: (TPO)294
Sequence: SDGRRTFPRI
Construct: SDGRR(pThr)FPRI (crude synthetic peptide)
Average holdup BI: 0.86
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 115
P value: 4.27
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.74
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 18

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 312-321
Site: 14-3-3_pThr317
Modification: (TPO)317
Sequence: SRSLSTNGEN
Construct: SRSLS(pThr)NGEN (crude synthetic peptide)
Average holdup BI: -0.1
Holdup BI standard deviation: 0.91
Immobilized partner concentration (10-6M): 115
P value: 0.08
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 19

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 511-520
Site: 14-3-3_pThr516
Modification: (TPO)516
Sequence: SKRLQTICMS
Construct: SKRLQ(pThr)ICMS (crude synthetic peptide)
Average holdup BI: 0.36
Holdup BI standard deviation: 0.5
Immobilized partner concentration (10-6M): 134
P value: 0.43
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 20

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 523-532
Site: 14-3-3_pThr528
Modification: (TPO)528
Sequence: GMRSVTGTPY
Construct: GMRSV(pThr)GTPY (crude synthetic peptide)
Average holdup BI: 0.82
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 134
P value: 5.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.54
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 21

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 523-532
Site: 14-3-3_pThr528
Modification: (TPO)528
Sequence: GMRSVTGTPY
Construct: GMRSV(pThr)GTPY (crude synthetic peptide)
Average holdup BI: 0.77
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 129
P value: 7.26
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.42
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 22

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MAP3K3
Fragment: 523-532
Site: 14-3-3_pThr528
Modification: (TPO)528
Sequence: GMRSVTGTPY
Construct: GMRSV(pThr)GTPY (crude synthetic peptide)
Average holdup BI: 0.79
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 115
P value: 4.79
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.52
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))