Interaction Details


Details of experiments between P61981 and Q6WCQ1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 1011-1020
Site: 14-3-3_pSer1016
Modification: (SEP)1016
Sequence: NIRSKSVIEQ
Construct: NIRSK(pSer)VIEQ (crude synthetic peptide)
Average holdup BI: 0.65
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 129
P value: 9.07
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.16
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 1011-1020
Site: 14-3-3_pSer1016
Modification: (SEP)1016
Sequence: NIRSKSVIEQ
Construct: NIRSK(pSer)VIEQ (crude synthetic peptide)
Average holdup BI: 0.56
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 134
P value: 4.99
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.97
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 3

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 1011-1020
Site: 14-3-3_pSer1016
Modification: (SEP)1016
Sequence: NIRSKSLKEG
Construct: NIRSK(pSer)LKEG (crude synthetic peptide)
Average holdup BI: -0.22
Holdup BI standard deviation: 1.16
Immobilized partner concentration (10-6M): 134
P value: 0.15
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 1011-1020
Site: 14-3-3_pSer1016
Modification: (SEP)1016
Sequence: NIRSKSLKEG
Construct: NIRSK(pSer)LKEG (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.71
Immobilized partner concentration (10-6M): 129
P value: 0.02
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 1011-1020
Site: 14-3-3_pSer1016
Modification: (SEP)1016
Sequence: NIRSKSVIEQ
Construct: NIRSK(pSer)VIEQ (crude synthetic peptide)
Average holdup BI: 0.58
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 115
P value: 5.41
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.09
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 264-273
Site: 14-3-3_pSer269
Modification: (SEP)269
Sequence: ESGYFSLEKT
Construct: ESGYF(pSer)LEKT (crude synthetic peptide)
Average holdup BI: 0.16
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 134
P value: 4.57
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 264-273
Site: 14-3-3_pSer269
Modification: (SEP)269
Sequence: ESGYFSLEKT
Construct: ESGYF(pSer)LEKT (crude synthetic peptide)
Average holdup BI: 0.22
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 129
P value: 3.84
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 8

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 264-273
Site: 14-3-3_pSer269
Modification: (SEP)269
Sequence: ESGYFSLEKT
Construct: ESGYF(pSer)LEKT (crude synthetic peptide)
Average holdup BI: 0.17
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 115
P value: 2.32
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 289-298
Site: 14-3-3_pSer294
Modification: (SEP)294
Sequence: SPPSPSTPNH
Construct: SPPSP(pSer)TPNH (crude synthetic peptide)
Average holdup BI: 0.22
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 134
P value: 3.7
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 289-298
Site: 14-3-3_pSer294
Modification: (SEP)294
Sequence: SPPSPSTPNH
Construct: SPPSP(pSer)TPNH (crude synthetic peptide)
Average holdup BI: 0.24
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 129
P value: 5.98
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 11

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 289-298
Site: 14-3-3_pSer294
Modification: (SEP)294
Sequence: SPPSPSTPNH
Construct: SPPSP(pSer)TPNH (crude synthetic peptide)
Average holdup BI: 0.16
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 115
P value: 2.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 12

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 296-305
Site: 14-3-3_pSer301
Modification: (SEP)301
Sequence: PNHRRSQVIE
Construct: PNHRR(pSer)QVIE (crude synthetic peptide)
Average holdup BI: -0.33
Holdup BI standard deviation: 0.15
Immobilized partner concentration (10-6M): 134
P value: 1.19
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 13

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 296-305
Site: 14-3-3_pSer301
Modification: (SEP)301
Sequence: PNHRRSQVIE
Construct: PNHRR(pSer)QVIE (crude synthetic peptide)
Average holdup BI: 0.2
Holdup BI standard deviation: 0.44
Immobilized partner concentration (10-6M): 129
P value: 0.26
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 14

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 296-305
Site: 14-3-3_pSer301
Modification: (SEP)301
Sequence: PNHRRSQVIE
Construct: PNHRR(pSer)QVIE (crude synthetic peptide)
Measured dissociation constant (10-6M): <100
Immobilized partner concentration (10-6M): 115
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 1
Measured pKd: 4
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 15

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 988-997
Site: 14-3-3_pSer993
Modification: (SEP)993
Sequence: IMKSKSNPDF
Construct: IMKSK(pSer)NPDF (crude synthetic peptide)
Average holdup BI: 1
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 134
P value: 3.92
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 6.43
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 16

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 988-997
Site: 14-3-3_pSer993
Modification: (SEP)993
Sequence: IMKSKSNPDF
Construct: IMKSK(pSer)NPDF (crude synthetic peptide)
Measured dissociation constant (10-6M): <1
Immobilized partner concentration (10-6M): 129
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 1
Measured pKd: 6
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 17

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 988-997
Site: 14-3-3_pSer993
Modification: (SEP)993
Sequence: IMKSKSNPDF
Construct: IMKSK(pSer)NPDF (crude synthetic peptide)
Measured dissociation constant (10-6M): <10
Immobilized partner concentration (10-6M): 115
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 1
Measured pKd: 5
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 18

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 373-382
Site: 14-3-3_pThr378
Modification: (TPO)378
Sequence: LDRRSTEPSV
Construct: LDRRS(pThr)EPSV (crude synthetic peptide)
Average holdup BI: 0.75
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 129
P value: 8.71
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.37
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 19

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 373-382
Site: 14-3-3_pThr378
Modification: (TPO)378
Sequence: LDRRSTEPSV
Construct: LDRRS(pThr)EPSV (crude synthetic peptide)
Average holdup BI: 0.73
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 134
P value: 6.76
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.31
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 20

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 373-382
Site: 14-3-3_pThr378
Modification: (TPO)378
Sequence: LDRRSTEPSV
Construct: LDRRS(pThr)EPSV (crude synthetic peptide)
Average holdup BI: 0.63
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 115
P value: 6.29
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.18
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 21

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 537-546
Site: 14-3-3_pThr542
Modification: (TPO)542
Sequence: EGRSKTFDWA
Construct: EGRSK(pThr)FDWA (crude synthetic peptide)
Average holdup BI: 0.75
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 134
P value: 6.03
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.35
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 22

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 537-546
Site: 14-3-3_pThr542
Modification: (TPO)542
Sequence: EGRSKTFDWA
Construct: EGRSK(pThr)FDWA (crude synthetic peptide)
Average holdup BI: 0.67
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 129
P value: 6.54
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.2
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 23

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: MPRIP
Fragment: 537-546
Site: 14-3-3_pThr542
Modification: (TPO)542
Sequence: EGRSKTFDWA
Construct: EGRSK(pThr)FDWA (crude synthetic peptide)
Average holdup BI: 0.59
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 115
P value: 7.17
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.12
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))