Interaction Details


Details of experiments between P61981 and Q5JSL3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 7-16
Site: 14-3-3_pSer12
Modification: (SEP)12
Sequence: FTKRLSKPGT
Construct: FTKRL(pSer)KPGT (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.14
Immobilized partner concentration (10-6M): 134
P value: 0.1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 7-16
Site: 14-3-3_pSer12
Modification: (SEP)12
Sequence: FTKRLSKPGT
Construct: FTKRL(pSer)KPGT (crude synthetic peptide)
Average holdup BI: 0.07
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 129
P value: 1.05
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 7-16
Site: 14-3-3_pSer12
Modification: (SEP)12
Sequence: FTKRLSKPGT
Construct: FTKRL(pSer)KPGT (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 115
P value: 0.46
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 1232-1241
Site: 14-3-3_pSer1237
Modification: (SEP)1237
Sequence: IKREDSRGSL
Construct: IKRED(pSer)RGSL (crude synthetic peptide)
Average holdup BI: 0.14
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 134
P value: 1.14
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 1232-1241
Site: 14-3-3_pSer1237
Modification: (SEP)1237
Sequence: IKREDSRGSL
Construct: IKRED(pSer)RGSL (crude synthetic peptide)
Average holdup BI: 0.53
Holdup BI standard deviation: 0.16
Immobilized partner concentration (10-6M): 115
P value: 2.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 324-333
Site: 14-3-3_pSer329
Modification: (SEP)329
Sequence: QLNKLSRGDG
Construct: QLNKL(pSer)RGDG (crude synthetic peptide)
Average holdup BI: -0.06
Holdup BI standard deviation: 0.25
Immobilized partner concentration (10-6M): 134
P value: 0.18
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 324-333
Site: 14-3-3_pSer329
Modification: (SEP)329
Sequence: QLNKLSRGDG
Construct: QLNKL(pSer)RGDG (crude synthetic peptide)
Average holdup BI: 0.1
Holdup BI standard deviation: 0.27
Immobilized partner concentration (10-6M): 129
P value: 0.31
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 8

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 324-333
Site: 14-3-3_pSer329
Modification: (SEP)329
Sequence: QLNKLSRGDG
Construct: QLNKL(pSer)RGDG (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.23
Immobilized partner concentration (10-6M): 115
P value: 0.08
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 495-504
Site: 14-3-3_pSer500
Modification: (SEP)500
Sequence: PYIKNSDPVK
Construct: PYIKN(pSer)DPVK (crude synthetic peptide)
Average holdup BI: 0.15
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 134
P value: 2.95
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 495-504
Site: 14-3-3_pSer500
Modification: (SEP)500
Sequence: PYIKNSDPVK
Construct: PYIKN(pSer)DPVK (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 129
P value: 0.21
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 11

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 495-504
Site: 14-3-3_pSer500
Modification: (SEP)500
Sequence: PYIKNSDPVK
Construct: PYIKN(pSer)DPVK (crude synthetic peptide)
Average holdup BI: 0.09
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 115
P value: 0.8
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 12

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 53-62
Site: 14-3-3_pSer58
Modification: (SEP)58
Sequence: KTQIYSDPLR
Construct: KTQIY(pSer)DPLR (crude synthetic peptide)
Average holdup BI: 0.93
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 129
P value: 7.78
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.02
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 13

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 53-62
Site: 14-3-3_pSer58
Modification: (SEP)58
Sequence: KTQIYSDPLR
Construct: KTQIY(pSer)DPLR (crude synthetic peptide)
Average holdup BI: 0.93
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 134
P value: 10.66
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.98
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 14

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 53-62
Site: 14-3-3_pSer58
Modification: (SEP)58
Sequence: KTQIYSDPLR
Construct: KTQIY(pSer)DPLR (crude synthetic peptide)
Average holdup BI: 0.9
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 115
P value: 7.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.9
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 15

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 1347-1356
Site: 14-3-3_pThr1352
Modification: (TPO)1352
Sequence: DRKSQTMPAL
Construct: DRKSQ(pThr)MPAL (crude synthetic peptide)
Measured dissociation constant (10-6M): <1
Immobilized partner concentration (10-6M): 129
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 1
Measured pKd: 6
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 16

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 1347-1356
Site: 14-3-3_pThr1352
Modification: (TPO)1352
Sequence: DRKSQTMPAL
Construct: DRKSQ(pThr)MPAL (crude synthetic peptide)
Average holdup BI: 0.97
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 134
P value: 5.41
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 5.35
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 17

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 82-91
Site: 14-3-3_pThr87
Modification: (TPO)87
Sequence: RTVQSTVPED
Construct: RTVQS(pThr)VPED (crude synthetic peptide)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 134
P value: 1.47
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 18

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 82-91
Site: 14-3-3_pThr87
Modification: (TPO)87
Sequence: RTVQSTVPED
Construct: RTVQS(pThr)VPED (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 129
P value: 0.83
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 19

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: DOCK11
Fragment: 82-91
Site: 14-3-3_pThr87
Modification: (TPO)87
Sequence: RTVQSTVPED
Construct: RTVQS(pThr)VPED (crude synthetic peptide)
Average holdup BI: 0.17
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 115
P value: 5.14
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))