Interaction Details

Details of experiments between P60484 and Q8TF64
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: GIPC3
Fragment: 107-202
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Measured dissociation constant (10^-6M):

Standard deviation of dissociation constant (10^-6M): 0

Average holdup BI: 0.03

Normalized holdup BI: 0

Normalized holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 17

P value: 0

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 33382810