Interaction Details

Details of experiments between P60484 and Q8NI35
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV

Protein B

Protein: InaDl
Fragment: 1425-1530
Site: PDZ_8
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.31

Normalized holdup BI: 0.33

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 4.47

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 1425-1530
Site: PDZ_8
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.25

Normalized holdup BI: 0.34

Immobilized partner concentration (10^-6M): 19

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 4.46

PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: InaDl
Fragment: 1425-1530
Site: PDZ_8
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.25

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

Measured pK_d: 4.31

PUBMED ID: 33382810

Experiment series 4

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 1425-1530
Site: PDZ_8
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.39

Immobilized partner concentration (10^-6M): 36

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

Measured pK_d: 4.26

PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 1058-1167
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.1

Holdup BI standard deviation: 0.01

Normalized holdup BI: 0.11

Normalized holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 19

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 2

Measured pK_d: 3.82

PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 113-229
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.1

Normalized holdup BI: 0.1

Immobilized partner concentration (10^-6M): 19

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.77

PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 1219-1332
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.14

Immobilized partner concentration (10^-6M): 36

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

Measured pK_d: 3.65

PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 1673-1763
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.07

Normalized holdup BI: 0.07

Immobilized partner concentration (10^-6M): 19

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.58

PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 359-469
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.05

Normalized holdup BI: 0.05

Immobilized partner concentration (10^-6M): 19

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.45

PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: InaDl
Fragment: 1058-1167
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.04

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 33382810

Experiment series 11

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: InaDl
Fragment: 113-229
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.09

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 33382810

Experiment series 12

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: InaDl
Fragment: 1219-1332
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.07

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 33382810

Experiment series 13

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: InaDl
Fragment: 1530-1615
Site: PDZ_9
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.06

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 33382810

Experiment series 14

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: InaDl
Fragment: 1673-1763
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.05

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 33382810

Experiment series 15

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: InaDl
Fragment: 238-335
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.04

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 33382810

Experiment series 16

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: InaDl
Fragment: 359-469
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.03

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 33382810

Experiment series 17

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: InaDl
Fragment: 547-651
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.08

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 33382810

Experiment series 18

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: InaDl
Fragment: 673-780
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.04

Immobilized partner concentration (10^-6M): 17

Experiment method: CALIP_holdup

Details of affinity fitting: lysozyme internal standard

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 33382810

Experiment series 19

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 1058-1167
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.08

Immobilized partner concentration (10^-6M): 36

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 20

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 113-229
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.02

Immobilized partner concentration (10^-6M): 36

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 21

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 1530-1615
Site: PDZ_9
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.01

Immobilized partner concentration (10^-6M): 36

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 22

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 1530-1615
Site: PDZ_9
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.04

Normalized holdup BI: 0.04

Immobilized partner concentration (10^-6M): 19

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 23

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 1673-1763
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.02

Immobilized partner concentration (10^-6M): 36

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 24

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 238-335
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.01

Immobilized partner concentration (10^-6M): 36

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 25

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 238-335
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.04

Normalized holdup BI: 0.04

Immobilized partner concentration (10^-6M): 19

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 26

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 359-469
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0

Immobilized partner concentration (10^-6M): 36

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 27

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 547-651
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.05

Immobilized partner concentration (10^-6M): 36

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 28

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 547-651
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.04

Normalized holdup BI: 0.04

Immobilized partner concentration (10^-6M): 19

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 29

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 673-780
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.04

Immobilized partner concentration (10^-6M): 36

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 30

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: Inadl
Fragment: 673-780
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.05

Normalized holdup BI: 0.05

Immobilized partner concentration (10^-6M): 19

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 31

Protein A

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV

Protein B

Protein: InaDl
Fragment: 1058-1167
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.05

Normalized holdup BI: 0.05

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 32

Protein A

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV

Protein B

Protein: InaDl
Fragment: 113-229
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.01

Normalized holdup BI: -0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 33

Protein A

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV

Protein B

Protein: InaDl
Fragment: 1530-1615
Site: PDZ_9
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.01

Normalized holdup BI: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 34

Protein A

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV

Protein B

Protein: InaDl
Fragment: 1673-1763
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0

Normalized holdup BI: -0

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 35

Protein A

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV

Protein B

Protein: InaDl
Fragment: 238-335
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.01

Normalized holdup BI: -0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 36

Protein A

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV

Protein B

Protein: InaDl
Fragment: 359-469
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0

Normalized holdup BI: 0

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 37

Protein A

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV

Protein B

Protein: InaDl
Fragment: 547-651
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.03

Normalized holdup BI: -0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 38

Protein A

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV

Protein B

Protein: InaDl
Fragment: 673-780
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.02

Normalized holdup BI: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 39

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 1425-1530
Site: PDZ_8
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.37

Normalized holdup BI: 0.5

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 4.82

PUBMED ID: 36115835

Experiment series 40

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 1425-1530
Site: PDZ_8
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.37

Normalized holdup BI: 0.4

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 4.64

Experimental Details

not reported internal standards of holdup layout #5

Experiment series 41

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 1425-1530
Site: PDZ_8
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.38

Normalized holdup BI: 0.4

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 4.61

PUBMED ID: 36115835

Experimental Details

not reported internal standards of holdup layout #6

Experiment series 42

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 1425-1530
Site: PDZ_8
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.51

Immobilized partner concentration (10^-6M): 29

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

Measured pK_d: 4.58

PUBMED ID: 36115835

Experiment series 43

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 1058-1167
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.24

Holdup BI standard deviation: 0

Normalized holdup BI: 0.27

Normalized holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 2

Measured pK_d: 4.37

PUBMED ID: 36115835

Experiment series 44

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 1058-1167
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.23

Normalized holdup BI: 0.25

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 4.33

Experimental Details

not reported internal standards of holdup layout #5

Experiment series 45

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 1058-1167
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.24

Normalized holdup BI: 0.24

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 4.26

PUBMED ID: 36115835

Experimental Details

not reported internal standards of holdup layout #6

Experiment series 46

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 1058-1167
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.32

Immobilized partner concentration (10^-6M): 29

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

Measured pK_d: 4.22

PUBMED ID: 36115835

Experiment series 47

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 1219-1332
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.31

Immobilized partner concentration (10^-6M): 29

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

Measured pK_d: 4.2

PUBMED ID: 36115835

Experiment series 48

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 113-229
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.1

Normalized holdup BI: 0.1

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.85

PUBMED ID: 36115835

Experiment series 49

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 359-469
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.09

Normalized holdup BI: 0.1

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.84

Experimental Details

not reported internal standards of holdup layout #5

Experiment series 50

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 238-335
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.08

Normalized holdup BI: 0.08

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.73

Experimental Details

not reported internal standards of holdup layout #5

Experiment series 51

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 1530-1615
Site: PDZ_9
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.07

Normalized holdup BI: 0.07

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.67

PUBMED ID: 36115835

Experiment series 52

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 238-335
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.07

Normalized holdup BI: 0.07

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.62

PUBMED ID: 36115835

Experimental Details

not reported internal standards of holdup layout #6

Experiment series 53

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 1530-1615
Site: PDZ_9
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.07

Normalized holdup BI: 0.07

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.62

Experimental Details

not reported internal standards of holdup layout #5

Experiment series 54

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 113-229
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.06

Normalized holdup BI: 0.06

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.62

Experimental Details

not reported internal standards of holdup layout #5

Experiment series 55

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 238-335
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.06

Normalized holdup BI: 0.06

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.6

PUBMED ID: 36115835

Experiment series 56

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 1673-1763
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.06

Normalized holdup BI: 0.06

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.57

Experimental Details

not reported internal standards of holdup layout #5

Experiment series 57

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 113-229
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.02

Normalized holdup BI: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

not reported internal standards of holdup layout #6

Experiment series 58

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 1530-1615
Site: PDZ_9
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.03

Normalized holdup BI: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

not reported internal standards of holdup layout #6

Experiment series 59

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 1673-1763
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.01

Normalized holdup BI: -0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

not reported internal standards of holdup layout #6

Experiment series 60

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 359-469
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.03

Normalized holdup BI: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

not reported internal standards of holdup layout #6

Experiment series 61

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 547-651
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.05

Normalized holdup BI: -0.05

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

not reported internal standards of holdup layout #6

Experiment series 62

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 547-651
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0

Normalized holdup BI: -0

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

Experimental Details

not reported internal standards of holdup layout #5

Experiment series 63

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 673-780
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.01

Normalized holdup BI: -0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

not reported internal standards of holdup layout #6

Experiment series 64

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: InaDl
Fragment: 673-780
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.01

Normalized holdup BI: 0.01

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

Experimental Details

not reported internal standards of holdup layout #5

Experiment series 65

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 113-229
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0

Immobilized partner concentration (10^-6M): 29

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 66

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 1530-1615
Site: PDZ_9
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.07

Immobilized partner concentration (10^-6M): 29

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 67

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 1673-1763
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0

Normalized holdup BI: -0

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 68

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 359-469
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.02

Immobilized partner concentration (10^-6M): 29

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 69

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 359-469
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.04

Normalized holdup BI: 0.04

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 70

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 547-651
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.11

Immobilized partner concentration (10^-6M): 29

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 71

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 547-651
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.07

Normalized holdup BI: -0.07

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 72

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 673-780
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.08

Immobilized partner concentration (10^-6M): 29

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 73

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: Inadl
Fragment: 673-780
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.01

Normalized holdup BI: 0.01

Immobilized partner concentration (10^-6M): 17

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835