Interaction Details


Details of experiments between P60484 and Q14160
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.51
Normalized holdup BI: 0.53
Immobilized partner concentration (10-6M): 19
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.83
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.17
Immobilized partner concentration (10-6M): 17
Experiment method: CALIP_holdup
Details of affinity fitting: lysozyme internal standard
Number of measurements: 1
Measured pKd: 4.11
PUBMED ID: 33382810

Experiment series 3

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Normalized holdup BI: 0.14
Immobilized partner concentration (10-6M): 19
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.94
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Holdup BI standard deviation: 0
Normalized holdup BI: 0.09
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 19
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.73
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.62
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.52
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 7

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 1090-1197
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 17
Experiment method: CALIP_holdup
Details of affinity fitting: lysozyme internal standard
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 33382810

Experiment series 8

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 17
Experiment method: CALIP_holdup
Details of affinity fitting: lysozyme internal standard
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 33382810

Experiment series 9

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.07
Immobilized partner concentration (10-6M): 17
Experiment method: CALIP_holdup
Details of affinity fitting: lysozyme internal standard
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 33382810

Experiment series 10

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 1090-1197
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 36
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 11

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 36
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 12

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 13

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.18
Immobilized partner concentration (10-6M): 36
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 14

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 36
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 15

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 16

Protein A

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 17

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.67
Normalized holdup BI: 0.71
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.23
PUBMED ID: 36115835

Experiment series 18

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.17
Normalized holdup BI: 0.17
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.07
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 19

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 131.59
Standard deviation of dissociation constant (10-6M): 5.06
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 3.88
PUBMED ID: 36115835

Experiment series 20

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 29
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 3.84
PUBMED ID: 36115835

Experiment series 21

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Normalized holdup BI: 0.1
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.83
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 22

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Normalized holdup BI: 0.09
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.79
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 23

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Normalized holdup BI: 0.08
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.74
PUBMED ID: 36115835

Experiment series 24

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Normalized holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.7
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 25

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.65
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 26

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.61
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 27

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SCRIB
Fragment: 1090-1197
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 29
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 28

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.04
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 29

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.05
Immobilized partner concentration (10-6M): 29
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 30

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 31

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835