Interaction Details


Details of experiments between P60484 and Q13424
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.15
Normalized holdup BI: 0.17
Immobilized partner concentration (10-6M): 19
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.04
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: PTEN
Fragment: 394-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ado-ado-EDQHTQITKV

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Normalized holdup BI: 0.15
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.02
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: PTEN
Fragment: 391-403
Site: PBM
Sequence: EDQHTQITKV
Construct: Biotin-ttds-PFDEDQHTQITKV

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 17
Experiment method: CALIP_holdup
Details of affinity fitting: lysozyme internal standard
Number of measurements: 1
Measured pKd: 4
PUBMED ID: 33382810

Experiment series 4

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 36
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 3.79
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKV

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 193.98
Standard deviation of dissociation constant (10-6M): 17.47
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 3.71
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.38
Normalized holdup BI: 0.44
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.71
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.32
Normalized holdup BI: 0.42
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.68
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 8

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.34
Normalized holdup BI: 0.38
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.57
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 9

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.41
Immobilized partner concentration (10-6M): 29
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 4.4
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: PTEN
Fragment: 393-403
Site: PBM
Modification: (ALY)402
Sequence: EDQHTQITKV
Construct: biotin-ttds-DEDQHTQITKacV

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 45.27
Standard deviation of dissociation constant (10-6M): 3.44
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.34
PUBMED ID: 36115835