Interaction Details


Details of experiments between P59637 and Q92743
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Protein B

Protein: HTRA1
Fragment: 365-480
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 21.83
Standard deviation of dissociation constant (10-6M): 1.57
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.66
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Protein B

Protein: HTRA1
Fragment: 365-480
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.4
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.57
PUBMED ID: 36115835