Interaction Details


Details of experiments between P59637 and Q8N448
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Protein B

Protein: LNX2
Fragment: 334-426
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.25
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.24
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Protein B

Protein: LNX2
Fragment: 592-688
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 3.74
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Protein B

Protein: LNX2
Fragment: 223-326
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.2
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Protein B

Protein: LNX2
Fragment: 459-560
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.11
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835