Interaction Details


Details of experiments between P59637 and O15018
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Protein B

Protein: PDZD2
Fragment: 2741-2839
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.26
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.26
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Protein B

Protein: PDZD2
Fragment: 2611-2718
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.07
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Protein B

Protein: PDZD2
Fragment: 326-424
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.19
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Protein B

Protein: PDZD2
Fragment: 326-424
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Protein B

Protein: PDZD2
Fragment: 575-684
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Protein B

Protein: PDZD2
Fragment: 64-181
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Protein B

Protein: PDZD2
Fragment: 722-815
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835