Interaction Details


Details of experiments between P57105 and Q6ZTQ3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.42
Immobilized partner concentration (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.99
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV
Average holdup BI: 0.53
Normalized holdup BI: 0.63
Immobilized partner concentration (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.97
PUBMED ID: 36115835