Interaction Details


Details of experiments between P57105 and Q05586
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: GRIN1
Fragment: 929-938
Site: PBM
Sequence: LSDPSVSTVV
Construct: Biotin-Ahx-LSDPSVSTVV
Average holdup BI: 0.07
Holdup BI standard deviation: 0.07
Normalized holdup BI: 0.08
Normalized holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.72
PUBMED ID: 36115835