Interaction Details


Details of experiments between P57105 and P06463
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV
Average holdup BI: 0.77
Normalized holdup BI: 0.81
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.46
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV
Measured dissociation constant (10-6M): 4.14
Standard deviation of dissociation constant (10-6M): 0.09
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 5.38
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV
Average holdup BI: 0.75
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.9
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 43
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.35
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV
Average holdup BI: 0.56
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 13.3
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 5.06
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV
Average holdup BI: 0.68
Normalized holdup BI: 0.81
Immobilized partner concentration (10-6M): 43
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.04
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 6

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV
Average holdup BI: 0.61
Normalized holdup BI: 0.76
Immobilized partner concentration (10-6M): 43
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.91
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 7

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: RLQRRRETQV
Construct: Biotin-ttds-RLQRRREpTQV
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835