Interaction Details


Details of experiments between P57105 and P03126
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Measured dissociation constant (10-6M): 3.5
Standard deviation of dissociation constant (10-6M): 0.08
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 5.46
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: Biotin-ado-ado-SSRTRRETQL
Average holdup BI: 0.69
Normalized holdup BI: 0.72
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.24
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.58
Holdup BI standard deviation: 0.07
Normalized holdup BI: 0.7
Normalized holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.18
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.65
Holdup BI standard deviation: 0.2
Immobilized partner concentration (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 8
Measured pKd: 5.18
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.54
Normalized holdup BI: 0.65
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.08
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 6

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.51
Normalized holdup BI: 0.64
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.06
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 7

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRRETQL
Measured dissociation constant (10-6M): 33
Standard deviation of dissociation constant (10-6M): 4
Experiment method: SPR
Number of measurements: 1
Measured pKd: 4.48
PUBMED ID: 30726710

Experiment series 8

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL
Average holdup BI: -0.05
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL
Measured dissociation constant (10-6M): 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835