Interaction Details


Details of experiments between P57105 and O43182
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGAP6
Fragment: 965-974
Site: PBM
Sequence: NPDALPETLV
Construct: biotin-ttds-NPDALPETLV
Average holdup BI: 0.14
Normalized holdup BI: 0.17
Immobilized partner concentration (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.06
PUBMED ID: 36115835