Interaction Details


Details of experiments between P56282 and Q96EL1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: POLE2
Fragment: 1-527
Construct: avi-his6-MBP-fusion protein

Protein B

Protein: INKA1
Fragment: 1-287
Construct: endogenous proteins (Jurkat)
Average holdup BI: 0.93
Immobilized partner concentration (10-6M): 26
P value: 8.48
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-02-02
Number of measurements: 6
Measured pKd: 5.7
Experimental Details

Batch = Helsinki_batch6; Ncontrols = 12; Nsamples = 6; Npreys = 6515; threshold curvature: 2.18