Interaction Details


Details of experiments between P56282 and O43426
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: POLE2
Fragment: 1-527
Construct: endogenous proteins (Jurkat)

Protein B

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2
Average holdup BI: 0.59
Immobilized partner concentration (10-6M): 10
P value: 3.29
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2023-12-11
Number of measurements: 6
Measured pKd: 5.15
Experimental Details

Batch = Helsinki_batch5; Ncontrols = 12; Nsamples = 6; Npreys = 6006; threshold curvature: 1.54

Experiment series 2

Protein A

Protein: POLE2
Fragment: 1-527
Construct: endogenous proteins (Jurkat)

Protein B

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2
Average holdup BI: 0.51
Immobilized partner concentration (10-6M): 10
P value: 8.15
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-04-11
Number of measurements: 6
Measured pKd: 5.03
Experimental Details

Batch = Helsinki_batch7; Ncontrols = 24; Nsamples = 6; Npreys = 6872; threshold curvature: 2.72

Experiment series 3

Protein A

Protein: POLE2
Fragment: 1-527
Construct: avi-his6-MBP-fusion protein

Protein B

Protein: SYNJ1
Fragment: 1-1573
Construct: endogenous proteins (Jurkat)
Average holdup BI: 0.43
Immobilized partner concentration (10-6M): 26
P value: 6.36
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-02-02
Number of measurements: 6
Measured pKd: 4.47
Experimental Details

Batch = Helsinki_batch6; Ncontrols = 12; Nsamples = 6; Npreys = 6515; threshold curvature: 2.18