Interaction Details


Details of experiments between P55345 and Q9ULL5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1-2036
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.92
Immobilized partner concentration (10-6M): 10
P value: 6.47
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 6.04
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1664-1678
Site: PxxP_5
Sequence: RKPWHRPPVPVRRSG
Construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.76
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1161-1175
Site: PxxP_10
Sequence: KAKRDGPPRPRGRPR
Construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Average holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.65
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1143-1157
Site: PxxP_9
Sequence: CPPNPGPDGPRRRGR
Construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1215-1229
Site: PxxP_1
Sequence: GGTRLEPLKPLKIKL
Construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1300-1314
Site: PxxP_3
Sequence: QAGLTPPLSPPKSVP
Construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Average holdup BI: -0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1309-1323
Site: PxxP_11
Sequence: PPKSVPPSVPARGLQ
Construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1453-1467
Site: PxxP_4
Sequence: LEEKPPPTPPPAPTP
Construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1691-1705
Site: PxxP_6
Sequence: APPPKAPAPPPKPET
Construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1697-1711
Site: PxxP_7
Sequence: PAPPPKPETPEKTTS
Construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 1720-1734
Site: PxxP_8
Sequence: ETAMPEPPAPEKPSL
Construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 986-1000
Site: PxxP_13
Sequence: AYDPYGPYCPGRASG
Construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 13-27
Site: PxxP_2
Modification: BAA86519.1
Sequence: HPVRPPPPPPPPMPL
Construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PRR12
Fragment: 5-19
Site: PxxP_12
Modification: BAA86519.1
Sequence: PGVPPGPPHPVRPPP
Construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680