Interaction Details

Details of experiments between P55345 and Q8IYB3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 395-409
Site: PxxP_3
Sequence: PRRRHRPSPPATPPP
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.29

Holdup BI standard deviation: 0.05

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 4.39

PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 395-409
Site: PxxP_3
Sequence: PRRRHRPSPPATPPP
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.29

Holdup BI standard deviation: 0.05

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 4.39

PUBMED ID: N

Experiment series 3

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 327-341
Site: PxxP_1
Sequence: TPPRRMPPPPRHRRS
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.26

Holdup BI standard deviation: 0.06

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 4.31

PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 327-341
Site: PxxP_1
Sequence: TPPRRMPPPPRHRRS
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.26

Holdup BI standard deviation: 0.06

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 4.31

PUBMED ID: N

Experiment series 5

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 544-558
Site: PxxP_4
Sequence: GRRRRSPSPPPTRRR
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.2

Holdup BI standard deviation: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 4.16

PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 544-558
Site: PxxP_4
Sequence: GRRRRSPSPPPTRRR
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.2

Holdup BI standard deviation: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 4.16

PUBMED ID: N

Experiment series 7

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 557-571
Site: PxxP_5
Sequence: RRRSPSPAPPPRRRR
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.18

Holdup BI standard deviation: 0.04

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 4.11

PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 557-571
Site: PxxP_5
Sequence: RRRSPSPAPPPRRRR
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.18

Holdup BI standard deviation: 0.04

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 4.11

PUBMED ID: N

Experiment series 9

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 567-581
Site: PxxP_6
Sequence: PRRRRTPTPPPRRRT
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.18

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 4

Measured pK_d: 4.09

PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 567-581
Site: PxxP_6
Sequence: PRRRRTPTPPPRRRT
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.18

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 4

Measured pK_d: 4.09

PUBMED ID: N

Experiment series 11

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 611-625
Site: PxxP_9
Sequence: KRRTASPPPPPKRRA
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.12

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 3.91

PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 611-625
Site: PxxP_9
Sequence: KRRTASPPPPPKRRA
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.12

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 3.91

PUBMED ID: N

Experiment series 13

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 612-626
Site: PxxP_10
Sequence: RRTASPPPPPKRRAS
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.12

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 3.89

PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 612-626
Site: PxxP_10
Sequence: RRTASPPPPPKRRAS
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.12

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

Measured pK_d: 3.89

PUBMED ID: N

Experiment series 15

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 576-590
Site: PxxP_7
Sequence: PPRRRTPSPPPRRRS
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.11

Holdup BI standard deviation: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 4

Measured pK_d: 3.84

PUBMED ID: 38995680

Experiment series 16

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 576-590
Site: PxxP_7
Sequence: PPRRRTPSPPPRRRS
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.11

Holdup BI standard deviation: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 4

Measured pK_d: 3.84

PUBMED ID: N

Experiment series 17

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 1-904
Construct: endogenous protein (Jurkat)

Average holdup BI: 0.03

Immobilized partner concentration (10^-6M): 10

P value: 1.17

Experiment method: nHU_Jurkat

Details of affinity fitting: hyperbolic binding equation

Date of measurement: 2022-06-29

Number of measurements: 3

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 18

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 365-379
Site: PxxP_2
Sequence: SRSRSPPKKPPKRTS
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.01

Holdup BI standard deviation: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 4

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 365-379
Site: PxxP_2
Sequence: SRSRSPPKKPPKRTS
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.01

Holdup BI standard deviation: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 4

The affinity was below the detection threshold of the assay.

PUBMED ID: N

Experiment series 20

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 576-590
Site: PxxP_7
Sequence: PPRRRTPSPPPRRRS
Construct: Bio E E Ado Ado-PPRRRTPSPPPRRRS-CONH2

Average holdup BI: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Details of affinity fitting: Quadratic binding equation

Date of measurement: 2023-01-16

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 21

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 600-614
Site: PxxP_8
Sequence: IQRRYSPSPPPKRRT
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.05

Holdup BI standard deviation: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 4

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 22

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 600-614
Site: PxxP_8
Sequence: IQRRYSPSPPPKRRT
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.05

Holdup BI standard deviation: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 4

The affinity was below the detection threshold of the assay.

PUBMED ID: N

Experiment series 23

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 621-635
Site: PxxP_11
Sequence: PKRRASPSPPPKRRV
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.04

Holdup BI standard deviation: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 4

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 24

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 621-635
Site: PxxP_11
Sequence: PKRRASPSPPPKRRV
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.04

Holdup BI standard deviation: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 4

The affinity was below the detection threshold of the assay.

PUBMED ID: N

Experiment series 25

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 759-773
Site: PxxP_12
Sequence: PAAKKPPAPPSPVQS
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.03

Holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 26

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 759-773
Site: PxxP_12
Sequence: PAAKKPPAPPSPVQS
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0.03

Holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: N

Experiment series 27

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 776-790
Site: PxxP_13
Sequence: PSTNWSPAVPVKKAK
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0

Holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 28

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: SRRM1
Fragment: 776-790
Site: PxxP_13
Sequence: PSTNWSPAVPVKKAK
Construct: Biotinylated 15-mer peptide (crude)

Average holdup BI: 0

Holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: N