Interaction Details


Details of experiments between P55345 and P23443
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: RPS6KB1
Fragment: 1-525
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.59
Immobilized partner concentration (10-6M): 10
P value: 5.01
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.15
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))