Interaction Details


Details of experiments between P55196 and Q14160
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MLLT4
Fragment: 528-541
Site: Internal_PBM
Construct: Biotin-GSGSGS-YGIVQETTFDLGGDIH-CONH2

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.34
Normalized holdup BI: 0.4
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.61
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MLLT4
Fragment: 528-541
Site: Internal_PBM
Construct: Biotin-GSGSGS-YGIVQETTFDLGGDIH-CONH2

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.25
Normalized holdup BI: 0.25
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.3
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MLLT4
Fragment: 528-541
Site: Internal_PBM
Construct: Biotin-GSGSGS-YGIVQETTFDLGGDIH-CONH2

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.65
PUBMED ID: 36115835