Interaction Details


Details of experiments between P55196 and O96018
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MLLT4
Fragment: 528-541
Site: Internal_PBM
Construct: Biotin-GSGSGS-YGIVQETTFDLGGDIH-CONH2

Protein B

Protein: APBA3
Fragment: 390-479
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.05
Normalized holdup BI: -0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835