Interaction Details


Details of experiments between P53778 and Q13424
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: Biotin-ado-ado-GARVSKETPL

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.39
Normalized holdup BI: 0.43
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.66
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: biotin-ttds-GARVSKETPL

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.29
Normalized holdup BI: 0.34
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.49
PUBMED ID: 36115835