Interaction Details


Details of experiments between P53671 and O75084
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LIMK2
Fragment: 143-248
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FZD7
Fragment: 565-574
Site: PBM
Sequence: SHSSKGETAV
Construct: biotin-ttds-SHSSKGETAV
Average holdup BI: 0.25
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.82
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: LIMK2
Fragment: 143-248
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FZD7
Fragment: 565-574
Site: PBM
Sequence: SHSSKGETAV
Construct: biotin-ttds-SHSSKGETAV
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835