Interaction Details


Details of experiments between P53671 and O00192
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LIMK2
Fragment: 143-248
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARVCF
Fragment: 953-962
Site: PBM
Sequence: AKPQPVDSWV
Construct: Biotin-Ahx-AKPQPVDSWV
Average holdup BI: -0.08
Normalized holdup BI: -0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835