Interaction Details


Details of experiments between P53667 and Q8N743
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A

Protein: LIMK1
Fragment: 156-267
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KIR3DL3
Fragment: 401-410
Site: PBM
Sequence: PKTPPTDTSV
Construct: biotin-ttds-PKTPPTDTSV
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835