Interaction Details


Details of experiments between P53667 and O15439
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LIMK1
Fragment: 156-267
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCC4
Fragment: 1316-1325
Site: PBM
Sequence: STLTIFETAL
Construct: biotin-ttds-STLTIFETAL
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.24
PUBMED ID: 36115835