Interaction Details


Details of experiments between P50479 and Q15418
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDLIM4
Fragment: 1-83
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPSTTL
Average holdup BI: -0.03
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 31
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: PDLIM4
Fragment: 1-83
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA1
Fragment: 725-735
Site: PBM
Modification: (SEP)732
Sequence: RVRKLPSTTL
Construct: biotin-ttds-RRVRKLPpSTTL
Average holdup BI: 0.14
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 40
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 3
Measured pKd: 3.63
PUBMED ID: 36115835