Interaction Details


Details of experiments between P50479 and Q02410
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDLIM4
Fragment: 321-330
Site: PBM
Sequence: PSASCAVTAA
Construct: Biotin-Ahx-PSASCAVTAA

Protein B

Protein: APBA1
Fragment: 655-741
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.08
Normalized holdup BI: -0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: PDLIM4
Fragment: 321-330
Site: PBM
Sequence: PSASCAVTAA
Construct: Biotin-Ahx-PSASCAVTAA

Protein B

Protein: APBA1
Fragment: 743-822
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: PDLIM4
Fragment: 237-246
Site: PBM
Modification: iso2
Sequence: VYPNAKVELV
Construct: biotin-ado-ado-VYPNAKVELV

Protein B

Protein: APBA1
Fragment: 743-822
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.11
Normalized holdup BI: 0.13
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.91
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 4

Protein A

Protein: PDLIM4
Fragment: 237-246
Site: PBM
Modification: iso2
Sequence: VYPNAKVELV
Construct: biotin-ado-ado-VYPNAKVELV

Protein B

Protein: APBA1
Fragment: 655-741
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.