Interaction Details

Details of experiments between P50479 and Q9Q6P4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: PDLIM4
Fragment: 1-83
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: 0.04

Holdup BI standard deviation: 0.1

Immobilized partner concentration (10^-6M): 18

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 5

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835