Interaction Details


Details of experiments between P50479 and P22460
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDLIM4
Fragment: 1-83
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNA5
Fragment: 604-613
Site: PBM
Sequence: CLDTSRETDL
Construct: biotin-ttds-CLDTSRETDL
Average holdup BI: 0.15
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.26
PUBMED ID: 36115835