Interaction Details

Details of experiments between P49796 and Q96KB5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: RGS3
Fragment: 300-378
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PBK
Fragment: 313-322
Site: PBM
Sequence: HIVEALETDV
Construct: biotin-ttds-HIVEALETDV

Measured dissociation constant (10^-6M):

Standard deviation of dissociation constant (10^-6M): 0

Average holdup BI: 0.04

Normalized holdup BI: 0

Normalized holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 5.5

P value: 0

Experiment method: SAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835