Interaction Details


Details of experiments between P49796 and P0DTC3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RGS3
Fragment: 300-378
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-Cov2-3a
Fragment: 266-275
Site: PBM
Sequence: EPTTTTSVPL
Construct: Biotin-(PEG)3-YDEPTTTTSVPL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.23
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 20
P value: 0
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.19
PUBMED ID: 36115835