Interaction Details


Details of experiments between P49418 and Q7Z5R6
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: APBB1IP
Fragment: 512-526
Site: PxxP_1
Sequence: PKSSLPPPPPVRRSS
Construct: Bio Ado Ado-PKSSLPPPPPVRRSS-CONH2
Average holdup BI: 0.15
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.01
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: APBB1IP
Fragment: 621-635
Site: PxxP_3
Sequence: PAVAKRPPVPPKRQE
Construct: Bio Ado Ado-PAVAKRPPVPPKRQE-CONH2
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.74
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: APBB1IP
Fragment: 622-636
Site: PxxP_4
Sequence: AVAKRPPVPPKRQEN
Construct: Bio Ado Ado-AVAKRPPVPPKRQEN-CONH2
Average holdup BI: 0.07
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 2
Measured pKd: 3.63
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: APBB1IP
Fragment: 1-666
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 10
P value: 1.39
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 5

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: APBB1IP
Fragment: 526-540
Site: PxxP_2
Sequence: SDTSGSPATPLKAKG
Construct: Bio Ado Ado-SDTSGSPATPLKAKG-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680