Interaction Details


Details of experiments between P49418 and Q5T200
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ZC3H13
Fragment: 1025-1039
Site: PxxP_1
Sequence: SKKKRGPRTPPITTK
Construct: Bio Ado Ado-SKKKRGPRTPPITTK-CONH2
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.74
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ZC3H13
Fragment: 562-576
Site: PxxP_3
Sequence: RSRGRVPELPEKGSR
Construct: Bio Ado Ado-RSRGRVPELPEKGSR-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.5
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: ZC3H13
Fragment: 1-1668
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.12
Immobilized partner concentration (10-6M): 10
P value: 3.57
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 4

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ZC3H13
Fragment: 255-269
Site: PxxP_2
Sequence: QSKKKGPRTPSPPPP
Construct: Bio Ado Ado-QSKKKGPRTPSPPPP-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ZC3H13
Fragment: 635-649
Site: PxxP_4
Sequence: RERDQRPSSPIRHQG
Construct: Bio Ado Ado-RERDQRPSSPIRHQG-CONH2
Average holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: ZC3H13
Fragment: 72-86
Site: PxxP_5
Sequence: SNYRRSPERPTGDLR
Construct: Bio Ado Ado-SNYRRSPERPTGDLR-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680