Interaction Details


Details of experiments between P49418 and Q01196
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: RUNX1
Fragment: 1-453
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.35
Immobilized partner concentration (10-6M): 10
P value: 6
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 4.73
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: RUNX1
Fragment: 167-181
Site: PxxP_4
Sequence: KITVDGPREPRRHRQ
Construct: Bio Ado Ado-KITVDGPREPRRHRQ-CONH2
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: RUNX1
Fragment: 207-221
Site: PxxP_5
Sequence: TAMRVSPHHPAPTPN
Construct: Bio Ado Ado-TAMRVSPHHPAPTPN-CONH2
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: RUNX1
Fragment: 268-282
Site: PxxP_6
Sequence: SVHPATPISPGRASG
Construct: Bio Ado Ado-SVHPATPISPGRASG-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: RUNX1
Fragment: 304-318
Site: PxxP_7
Sequence: SDPRQFPALPSISDP
Construct: Bio Ado Ado-SDPRQFPALPSISDP-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680