Interaction Details


Details of experiments between P49418 and P50570
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 1-870
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.65
Immobilized partner concentration (10-6M): 10
P value: 8.6
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.28
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 823-837
Site: PxxP_3
Sequence: DLFPAPPQIPSRPVR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.6
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.99
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 823-837
Site: PxxP_3
Sequence: DLFPAPPQIPSRPVR
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.6
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.99
PUBMED ID: N

Experiment series 4

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 826-840
Site: PxxP_4
Sequence: PAPPQIPSRPVRIPP
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.56
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.91
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 826-840
Site: PxxP_4
Sequence: PAPPQIPSRPVRIPP
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.56
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.91
PUBMED ID: N

Experiment series 6

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 826-840
Site: PxxP_4
Sequence: PAPPQIPSRPVRIPP
Construct: Bio E E Ado Ado-PAPPQIPSRPVRIPP-CONH2
Average holdup BI: 0.5
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.8
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 801-815
Site: PxxP_2
Sequence: AASFSAPPIPSRPGP
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.2
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.16
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 801-815
Site: PxxP_2
Sequence: AASFSAPPIPSRPGP
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.2
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.16
PUBMED ID: N

Experiment series 9

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 846-860
Site: PxxP_7
Sequence: VPSRRPPAAPSRPTI
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.15
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 4.01
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 846-860
Site: PxxP_7
Sequence: VPSRRPPAAPSRPTI
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.15
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 4.01
PUBMED ID: N

Experiment series 11

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 838-852
Site: PxxP_6
Sequence: IPPGIPPGVPSRRPP
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.14
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.97
PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 838-852
Site: PxxP_6
Sequence: IPPGIPPGVPSRRPP
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.14
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.97
PUBMED ID: N

Experiment series 13

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 838-852
Site: PxxP_6
Sequence: IPPGIPPGVPSRRPP
Construct: Bio E E Ado Ado-IPPGIPPGVPSRRPP-CONH2
Average holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.84
PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 313-327
Site: PxxP_1
Sequence: EYKNFRPDDPTRKTK
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: -0
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 15

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 313-327
Site: PxxP_1
Sequence: EYKNFRPDDPTRKTK
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: -0
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 16

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 834-848
Site: PxxP_5
Sequence: RPVRIPPGIPPGVPS
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.02
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 17

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: DNM2
Fragment: 834-848
Site: PxxP_5
Sequence: RPVRIPPGIPPGVPS
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.02
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N