Interaction Details


Details of experiments between P49418 and P30260
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.61
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 5
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.61
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 5
PUBMED ID: N

Experiment series 3

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: CDC27
Fragment: 1-824
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.48
Immobilized partner concentration (10-6M): 10
P value: 9.19
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 4.97
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 4

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: CDC27
Fragment: 396-410
Site: PxxP_1
Sequence: LKMKFPPKIPNRKTK
Construct: Bio E E Ado Ado-LKMKFPPKIPNRKTK-CONH2
Average holdup BI: 0.47
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.74
PUBMED ID: 38995680