Interaction Details


Details of experiments between P49418 and O14974
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: PPP1R12A
Fragment: 1-1030
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.27
Immobilized partner concentration (10-6M): 10
P value: 5.66
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 4.56
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.37
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.54
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: PPP1R12A
Fragment: 493-507
Site: PxxP_2
Sequence: RLAYVAPTIPRRLAS
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.37
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.54
PUBMED ID: N

Experiment series 4

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.02
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: PPP1R12A
Fragment: 401-415
Site: PxxP_1
Sequence: SSGQATPTSPIKKFP
Construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.02
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N