Interaction Details


Details of experiments between P47775 and P26045
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GPR12
Fragment: 325-334
Site: PBM
Sequence: AQRARSPSDV
Construct: biotin-ado-ado-AQRARSPSDV

Protein B

Protein: PTPN3
Fragment: 505-597
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Normalized holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.98
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.