Interaction Details


Details of experiments between P46095 and Q8TEW8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GPR6
Fragment: 353-362
Site: PBM
Sequence: PFRSRSPSEV
Construct: biotin-ttds-PFRSRSPSEV

Protein B

Protein: PARD3B
Fragment: 184-299
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Normalized holdup BI: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.8
PUBMED ID: 36115835