Interaction Details


Details of experiments between P42768 and Q5VST9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: WAS
Fragment: 374-388
Site: PxxP_7
Sequence: ATGRSGPLPPPPPGA
Construct: Bio Ado Ado-ATGRSGPLPPPPPGA-CONH2

Protein B

Protein: OBSCN
Fragment: 5594-5674
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.51
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: WAS
Fragment: 1-502
Construct: endogenous protein (Jurkat)

Protein B

Protein: OBSCN
Fragment: 5594-5674
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 10
P value: 0.64
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 3

Protein A

Protein: WAS
Fragment: 154-168
Site: PxxP_1
Sequence: GDRRQLPPPPTPANE
Construct: Bio Ado Ado-GDRRQLPPPPTPANE-CONH2

Protein B

Protein: OBSCN
Fragment: 5594-5674
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: WAS
Fragment: 306-320
Site: PxxP_2
Sequence: EMRRQEPLPPPPPPS
Construct: Bio Ado Ado-EMRRQEPLPPPPPPS-CONH2

Protein B

Protein: OBSCN
Fragment: 5594-5674
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: WAS
Fragment: 310-324
Site: PxxP_3
Sequence: QEPLPPPPPPSRGGN
Construct: Bio Ado Ado-QEPLPPPPPPSRGGN-CONH2

Protein B

Protein: OBSCN
Fragment: 5594-5674
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: WAS
Fragment: 335-349
Site: PxxP_4
Sequence: NKGRSGPLPPVPLGI
Construct: Bio Ado Ado-NKGRSGPLPPVPLGI-CONH2

Protein B

Protein: OBSCN
Fragment: 5594-5674
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: WAS
Fragment: 353-367
Site: PxxP_5
Sequence: PPTPRGPPPPGRGGP
Construct: Bio Ado Ado-PPTPRGPPPPGRGGP-CONH2

Protein B

Protein: OBSCN
Fragment: 5594-5674
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.09
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: WAS
Fragment: 361-375
Site: PxxP_6
Sequence: PPGRGGPPPPPPPAT
Construct: Bio Ado Ado-PPGRGGPPPPPPPAT-CONH2

Protein B

Protein: OBSCN
Fragment: 5594-5674
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680