Interaction Details


Details of experiments between P36809 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.58
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.9
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.81
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0.81
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 3
Measured pKd: 5.64
PUBMED ID: 36115835