Interaction Details


Details of experiments between P36809 and Q86UL8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 8.19
Standard deviation of dissociation constant (10-6M): 0.64
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 5.09
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.5
Normalized holdup BI: 0.55
Immobilized partner concentration (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.04
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.24
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.01
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.26
Normalized holdup BI: 0.31
Immobilized partner concentration (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.57
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.48
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.21
Normalized holdup BI: 0.21
Immobilized partner concentration (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.31
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Normalized holdup BI: 0.16
Immobilized partner concentration (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.19
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.09
Normalized holdup BI: 0.13
Immobilized partner concentration (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.07
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: MAGI2
Fragment: 1-102
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 11

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 12

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835