Interaction Details


Details of experiments between P36809 and Q14160
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.47
Normalized holdup BI: 0.59
Immobilized partner concentration (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.11
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.25
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.06
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.49
Normalized holdup BI: 0.54
Immobilized partner concentration (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.02
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.39
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.41
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 3
Measured pKd: 4.76
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 19.68
Standard deviation of dissociation constant (10-6M): 1.2
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.71
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.15
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.7
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: SCRIB
Fragment: 1090-1197
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.59
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.49
PUBMED ID: 36115835