Interaction Details


Details of experiments between P36762 and Q5TCQ9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV49-L2
Fragment: 512-521
Site: PBM
Sequence: RRRKRKRTYL
Construct: Biotin-Ahx-RRRKRKRTYL

Protein B

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.72
Normalized holdup BI: 0.79
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.4
PUBMED ID: 36115835