Interaction Details

Details of experiments between P36507 and Q9UMS6
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: MAP2K2
Fragment: 391-400
Site: PBM
Sequence: QPGTPTRTAV
Construct: biotin-ttds-QPGTPTRTAV

Protein B

Protein: SYNP2
Fragment: 2-90
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.03

Immobilized partner concentration (10^-6M): 10

Experiment method: SAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normal layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835