Interaction Details


Details of experiments between P36507 and Q12959
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAP2K2
Fragment: 1-400
Construct: endogenous protein (Jurkat)

Protein B

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.32
Immobilized partner concentration (10-6M): 10
P value: 4.61
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 4.67
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: MAP2K2
Fragment: 391-400
Site: PBM
Sequence: QPGTPTRTAV
Construct: biotin-ttds-QPGTPTRTAV

Protein B

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Normalized holdup BI: 0.15
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.99
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MAP2K2
Fragment: 391-400
Site: PBM
Sequence: QPGTPTRTAV
Construct: biotin-ttds-QPGTPTRTAV

Protein B

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.53
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MAP2K2
Fragment: 1-400
Construct: endogenous protein (Jurkat)

Protein B

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Immobilized partner concentration (10-6M): 10
P value: 1.54
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 5

Protein A

Protein: MAP2K2
Fragment: 391-400
Site: PBM
Sequence: QPGTPTRTAV
Construct: biotin-ttds-QPGTPTRTAV

Protein B

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: MAP2K2
Fragment: 391-400
Site: PBM
Sequence: QPGTPTRTAV
Construct: biotin-ttds-QPGTPTRTAV

Protein B

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: MAP2K2
Fragment: 391-400
Site: PBM
Sequence: QPGTPTRTAV
Construct: biotin-ttds-QPGTPTRTAV

Protein B

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.07
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: MAP2K2
Fragment: 391-400
Site: PBM
Sequence: QPGTPTRTAV
Construct: biotin-ttds-QPGTPTRTAV

Protein B

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835